Normal human peripheral blood cells were unstained (left) or stained with CD16 Monoclonal Antibody, NovaFluor Red 725 (right). All cells were co-stained with CD56 (NCAM) Monoclonal Antibody, eFluor 450 (Product # 48-0566-42). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.